Research Technician III - Donald K. Johnson Eye Institute

Union: Non-union Number of vacancies: 1 New or Replacement Position: New Site: Toronto Western Hospital Department: Donald K. Johnson Eye Institute Reports to: Principal Investigator Salary Range : $37.47 - $46.84 per hour Hours: 37.5 hours per week Status: Temporary Full-Time Closing Date: November 4, 2026 Position Summary The Research Technician III will play a key role in supporting innovative neuroscience and vision research within the Principal Investigator’s laboratory at the Donald K. Johnson Eye Institute and Krembil Research Institute. Working as part of a multidisciplinary research team, the incumbent will maintain and characterize human multipotent and pluripotent stem cell cultures, support the development of advanced 3D organoid models of the brain and eye, and perform a range of molecular and cellular biology techniques to advance studies of neurological and retinal disease. Duties • Maintaining cell lines (cultured and primary), including human multipotent and pluripotent stem cell cultures, using standard sterile techniques • Conduct experiments to support ongoing lab research projects (Western blots, qRT-PCR, immunocytochemistry and immunohistochemistry) • Management of tissue culture facilities, ordering supplies, lab management • Support the day-to-day operations of the laboratory, including stock preparation, supplies ordering, and general lab maintenance • Assist with biosafety compliance, including biological/chemical inventory management • Help develop standard operating procedures and protocols for lab-related activities • Attend and present data in weekly lab meeting discussions, institutional research seminars, and research conferences • Some administrative support to the PI • At minimum, a Bachelors degree in biological sciences, or equivalent discipline is required • At minimum, 6 years of related experience is required • Prior experience with multipotent and pluripotent stem cell cultures is required • 3D organoid culture experience is preferred • Prior experience with standard laboratory protocols for cell culture, Western blot, qRT-PCR, DNA electrophoresis and genotyping, molecular cloning • Prior experience with primary cell isolation from tissue specimens (animals or human) is an asset • Viral transduction and genetic engineering / gene-editing (e.g., CRISPR/Cas9) experience is an asset • RNA sequencing and bioinformatics experience is an asset • Willing to rapidly learn new techniques and skills with training • Capacity to work independently with minimal supervision • Highly-organized and able to prioritize effectively • Able to communicate and interact with staff, students, colleagues in a professional manner and daily basis (on-site) Why Join UHN? In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN. • Competitive offer packages • Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/ ) • Close access to Transit and UHN shuttle service • A flexible work environment • Opportunities for development and promotions within a large organization • Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.) Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.